Protein A/G Magnetic Beads from MedChemExpress

Product Specs
ItemProtein A/G Magnetic Beads
CompanyMedChemExpress
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberHY-K0202
Quantity1 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
TypeMagnetic Beads

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(1) hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoproteinVirulenceDecember 31, 2023An-Qi Liu, Xiangmou Qin, Hui Wu, Hao Feng, Yong-An Zhang, Jiagang Tuor anti-EGFP antibody according to the manufacturer’s instructions of protein A/G (MedChemExpress, HY-K0202). The eluted samples were then boiled with 2×SDS buffer for 10 min, followed by Western blotting. Nuclear

(2) Didymin alleviates metabolic dysfunction-associated fatty liver disease (MAFLD) via the stimulation of Sirt1-mediated lipophagy and mitochondrial biogenesisJ Transl MedDecember 19, 2023Jing-Wen Yang, Ying Zou, Jun Chen, Chen Cui, Jia Song, Meng-Meng Yang, Jing Gao, Hui-Qing Hu, Long-Qing Xia, Li-Ming Wang, et al.(https://biosys.bgi.com). Immunoprecipitation Protein A/G magnetic beads (MedChemExpress, New Jersey, USA, HY-K0202) were washed three times with RIPA lysis buffer (Beyotime, Shanghai, China, P0013B) three times before

(3) Cancer-associated fibroblasts-derived CXCL12 enhances immune escape of bladder cancer through inhibiting P62-mediated autophagic degradation of PDL1Journal of Experimental & Clinical Cancer ResearchNovember 25, 2023Zhao Zhang, Yongbo Yu, Zhilei Zhang, Dan Li, Zhijuan Liang, Liping Wang, Yuanbin Chen, Ye Liang, Haitao Niuof protein lysate were incubated overnight at 4 °C with antibodies and protein A/G magnetic beads (HY-K0202, MedChemExpress, Monmouth Junction, NJ, USA). Then the beads were washed at least three times with lysis

(4) The m7G Reader NCBP2 Promotes Pancreatic Cancer Progression by Upregulating MAPK/ERK SignalingCancers (Basel)November 17, 2023Jiancong Xie, Taiwei Mo, Ruibing Li, Hao Zhang, Guanzhan Liang, Tao Ma, Jing Chen, Hanlin Xie, Xiaofeng Wen, Tuo Hu, et al.(BioVision, San Francisco, CA, USA) conjugated with Protein A/G Magnetic Beads (MedChemExpress, MCE HY-K0202-5 mL, Monmouth Junction, NJ, USA). After elution, reverse transcription, and amplification, qPCR was

(5) PRMT3‐Mediated Arginine Methylation of METTL14 Promotes Malignant Progression and Treatment Resistance in Endometrial CarcinomaAdvanced ScienceNovember 16, 2023Yiru Wang, Can Wang, Xue Guan, Ying Ma, Shijie Zhang, Fei Li, Yue Yin, Zhenxing Sun, Xiuwei Chen, Hang Yin(Japan). Coimmunoprecipitation Assay Cell lysates were processed using A/G Magnetic Beads (MedChemExpress, HY‐K0202) as recommended by the manufacturer. The PRMT3 antibody, METTL14 antibody, Flag antibody, and IgG antibody

(6) Vitexin attenuates chronic kidney disease by inhibiting renal tubular epithelial cell ferroptosis via NRF2 activationMolecular MedicineOctober 27, 2023Jiayu Song, Hongri Wang, Jingyi Sheng, Wen Zhang, Juan Lei, Weihua Gan, Fangfang Cai, Yunwen Yangevaluated using ImageJ (NIH, USA). The co-IP assay was conducted with protein A/G beads (MedChemExpress, HY-K0202) as indicated by the instructions. In brief, cells were lysed in co-IP lysis buffer. The supernatant

(7) The brain protection of MLKL inhibitor necrosulfonamide against focal ischemia/reperfusion injury associating with blocking the nucleus and nuclear envelope translocation of MLKL and RIP3KFrontiers in PharmacologyOctober 24, 2023Xian-Yong Zhou, Bo Lin, Wei Chen, Rui-Qi Cao, Yi Guo, Ali Said, Taous Khan, Hui-Ling Zhang, Yong-Ming Zhuremoved and the pellet was stored. Co-immunoprecipitation Co-immunoprecipitation was performed using Protein A/G Magnetic Beads (MedChemExpress). Briefly, anti-MLKL antibody was added to the beads and incubated with rotation for

(8) Immunoglobulin G inhibits glucocorticoid-induced osteoporosis through occupation of FcγRIiScienceOctober 20, 2023Lijuan Jiang, Wenlin Qiu, Xuefei Wang, Xiaoru Duan, Xiaoxiao Han, Tong Yu, Shenghui Wen, Zhijun Luo, Ruizhi Feng, Yao Teng, et al.Kit,Sigma-Aldrich,387A Melon™ Gel IgG Purification Kit,Thermo Fisher,45212 Protein A/G magnetic beads,MedChemexpress,HY-K0202 Total RNA Extraction Reagent,Vazyme,R401–01 HiScript ® III-RT SuperMix,Vazyme,R323–01 SYBR® Green

(9) PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stabilityCell Death DiscoveryOctober 3, 2023Yuchong Peng, Youhong Liu, Rirong Zheng, Yubing Ye, Yongming Fu, Linglong Yin, Yingxue Gao, Yuxin Fu, Xuli Qi, Tanggang Deng, et al.incubated with the indicated antibodies on a rotator at 4 °C for 12 h, followed by incubating with protein A/G-magnetic beads (MedChemExpress, Shanghai, China) on a rotator at 4 °C for 1 h. After incubation, beads were washed

(10) SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate ShuttleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2023She X, Wu Q, Rao Z, Song D, Huang C, Feng S, Liu A, Liu L, Wan K, Li X, et al.anti‐HA magnetic beads (HY‐K0201), anti‐Flag magnetic beads (HY‐K0207), and Protein A/G magnetic beads (HY‐K0202) were purchased from MedChemExpress. EBSS (C0213), puromycin dihydrochloride (ST551), and G418 (ST081)

(11) Ischemia-reperfusion injury in human AC16 cardiomyocytes is modulated by AXIN1 depending on c-Myc regulationAnnals of Medicine & SurgeryOctober 1, 2023Jun Li, Hao Wang, Li Chen, Jialin Zhong, Junsheng Wang, Jun Xiaomeantime, a mixture of an antibody against AXIN1 (Abcam) or isotype IgG antibody (ab172730, Abcam) and protein A/G magnetic beads (MedChemExpress) was prepared and incubated for 4 h at 4°C. Following the cross-linking unfastening

(12) African Swine Fever Virus pF778R Attenuates Type I Interferon Response by Impeding STAT1 Nuclear TranslocationVirus ResearchOctober 1, 2023Qichao Chen, Liang Li, Lixinjie Liu, Zhankui Liu, Shibang Guo, Chen Tan, Huanchun Chen, Xiangru Wang4 °C overnight with rotation. Subsequently, the samples were immunoprecipitated via incubation with protein A/G magnetic beads (MedChemExpress, Monmouth Junction, NJ, USA) for 4 h and denatured by boiling in sodium dodecyl sulfate

(13) Use of Deep‐Learning Assisted Assessment of Cardiac Parameters in Zebrafish to Discover Cyanidin Chloride as a Novel Keap1 Inhibitor Against Doxorubicin‐Induced CardiotoxicityAdvanced ScienceOctober 1, 2023Changtong Liu, Yingchao Wang, Yixin Zeng, Zirong Kang, Hong Zhao, Kun Qi, Hongzhi Wu, Lu Zhao, Yi Wangwith indicated anti‐Keap1 or anti‐IgG antibody (1:200) at 4 °C overnight. 30 µL of protein A/G beads (HY‐K0202, MedChemExpress, USA) were added for another 2 h on a rotator. Immunoprecipitants were centrifuged and

(14) STAT5 promotes PD-L1 expression by facilitating histone lactylation to drive immunosuppression in acute myeloid leukemiaSignal Transduction and Targeted TherapySeptember 30, 2023Ze-Wei Huang, Xue-Ning Zhang, Ling Zhang, Ling-Ling Liu, Jing-Wen Zhang, Yu-Xiang Sun, Jue-Qiong Xu, Quentin Liu, Zi-Jie Longdialysis, the supernatant was collected and incubated with protein A/G magnetic beads (MedChemExpress, HY-K0202) which were preincubated with antibody at 4 °C overnight. Then DNA was collected by HiPure PCR Pure

(15) Activation of AMPK ameliorates acute severe pancreatitis by suppressing pancreatic acinar cell necroptosis in obese mice modelsCell Death DiscoverySeptember 30, 2023Kunlei Wang, Anbang Zhao, Dilinigeer Tayier, Kai Tan, Wenjing Song, Qian Cheng, Xinyin Li, Zhinan Chen, Qifeng Wei, Yufeng Yuan, et al.cell lysate was collected and used 10% of protein supernatant as input. Magnetic beads protein A/G (HY-K0202, MedChemExpress, China) were used to isolated non-specific binding proteins in the rest supernatant.

(16) Unimolecular Self-Assembled Hemicyanine–Oleic Acid Conjugate Acts as a Novel Succinate Dehydrogenase Inhibitor to Amplify Photodynamic Therapy and Eliminate Cancer Stem CellsResearch (Wash D C)September 6, 2023Qiang Wang, Tian Yang, Shiyou Li, Chen Xu, Chong Wang, Yuxuan Xiong, Xing Wang, Jiangling Wan, Xiangliang Yang, Zifu Li(Shanghai, China). Lyso-Tracker Red, Mito-Tracker green, ATP Assay Kit, and Immunoprecipitation Kit with Protein A+G Magnetic Beads were purchased from Beyotime Biotechnology (Shanghai, China). DCFDA was obtained from MedChemExpress

(17) Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cellsExperimental Hematology & OncologyAugust 29, 2023Juan-Juan Wang, Ding-Xiong Chen, Yu Zhang, Xin Xu, Yan Cai, Wen-Qiang Wei, Jia-Jie Hao, Ming-Rong Wang1: Table S7. Coimmunoprecipitation-based mass spectrometry (Co-IP-MS) Protein A/G magnetic beads (HY-K0202, MedChemExpress) were used to perform coimmunoprecipitation. Protein samples were then subjected to

(18) NEK8 regulates colorectal cancer progression via phosphorylating MYCCell Commun SignalAugust 18, 2023Beibei Cao, Kailun Zhang, Changjie Pan, Yifei Dong, Feng Luwere produced by ABclonal company. Anti-Flag Magnetic Beads (HY-K0207) and Protein A/G Magnetic Beads (HY-K0202) were purchased from MedChemExpress company. Western blot assays Pre-treated cells were lysed on ice

(19) Fibulin7 Mediated Pathological Cardiac Remodeling through EGFR Binding and EGFR‐Dependent FAK/AKT Signaling ActivationAdvanced ScienceAugust 1, 2023Xuehui Zheng, Lingxin Liu, Jing Liu, Chen Zhang, Jie Zhang, Yan Qi, Lin Xie, Chunmei Zhang, Guoqing Yao, Peili BuImmunoprecipitation (IP)‐grade antibodies or IgG for 1 h, followed by the addition of pretreated protein A/G magnetic beads (HY‐K0202; MedChemExpress, Monmouth Junction, NJ, USA) to a rotating wheel at 4°C overnight. The next day, the

(20) PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγDiabetesAugust 1, 2023Zhong Y, Wang Y, Li X, Qin H, Yan S, Rao C, Fan D, Liu D, Deng F, Miao Y, et al.antibodies or IgG (Cell Signaling Technology, 3900 and 5415), protein A/G magnetic beads (MedChemExpress, HY-K0202) were added to the protein samples, which were then incubated for an additional 2 h on a rotator. The

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Protein A/G Magnetic Beads from MedChemExpress

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